Opera Phenix™ Plus

Overview

The Opera Phenix Plus by Revvity is an advanced high-content screening platform for high-speed, high-sensitivity and highly automated imaging. It is especially suited for complex biological models such as organoids, 3D spheroids and microtissues, and supports applications including cell cycle studies, migration and invasion assays, cell signaling analysis, mitochondrial localization, membrane analysis, and fixed or live-cell imaging in thick tissues. Its combination of confocal spinning-disk imaging, automation and multiwell plate handling makes it a powerful tool for modern cell-based discovery workflows.

Research Applications

This system can be used in a wide variety of biological and biomedical research areas. It is particularly well suited for drug discovery and pharmacological screening, toxicology studies, cancer research, cell biology, neuroscience and infectious disease research. Typical applications include identifying compounds that affect cellular viability or function, monitoring proliferation, apoptosis, migration or invasion, studying organelle dynamics and cellular differentiation, analyzing neuronal morphology and synapse formation, and investigating host-pathogen interactions and immune responses.

Technical Specifications

-Fully integrated high-content screening solution

-High-speed confocal imaging using spinning-disk technology

-Supports 3D, multiplexed and time-lapse imaging

-Robotic loading system for automated handling of multiwell plates

-Compatible with plates from external racks or an integrated incubator

-Precise environmental control of temperature, CO₂ and humidity for live-cell experiments

-Built-in liquid handling system for simultaneous experimental manipulation and acquisition

-Laser-based autofocus for rapid and accurate confocal imaging across wells and plates

-Real-time image analysis capabilities for event detection and identification of cells or objects of interest