Leica Thunder

Overview

The Leica Thunder is an advanced fluorescence microscope for the study of cell cultures, 3D tissue assays, organoids, embryos and related samples. It expands and improves the imaging capabilities previously offered by the Leica DMIRE2 fluorescence microscope. The system combines full automation with computational image clearing, allowing users to generate sharper fluorescence images in thick samples and large fields of view. It is particularly useful as a complementary tool to confocal microscopy when high-quality widefield fluorescence imaging is needed.

Research Applications

The Leica Thunder is suitable for imaging whole tissues and large mosaics with up to four fluorescent markers, as well as for quantification and analysis of cellular markers in large regions such as whole coverslips. It also supports screening of markers in multiwell plates. Because it provides high-quality fluorescence imaging with reduced out-of-focus blur, it is a valuable system for tissue imaging, embryo and organoid studies, and other applications requiring rapid acquisition over broad sample areas.

Technical Specifications

-Fully automated system with motorized control of stage position, focus and fluorescence illumination

-Highly sensitive sCMOS camera with 90% quantum efficiency

-Large field of view of 18 mm diagonal for rapid capture of large mosaics

-LED light source with eight lines and broad fluorescence dye compatibility across the 395–730 nm range

-Built-in computational clearing and deconvolution tools through Leica Thunder technology

-Reassigns light to its correct focal plane, producing images comparable to confocal microscopy in many applications