Ultima 2Pplus

Overview

The Ultima 2Pplus by Bruker is an advanced multiphoton microscope designed to significantly expand high-resolution optical imaging in live samples. It enables precise and detailed imaging at the cellular, subcellular and molecular levels, particularly in living tissues and animal models. The system is optimized for in vivo fluorescence imaging and supports demanding applications in neuroscience and other biomedical fields. It also enables functional experiments such as optogenetic photostimulation and glutamate uncaging, as well as photodynamic and photochemical assays.

Research Applications

The system is used for high-resolution in vivo imaging in fluorescent animal models or in samples labeled with intravascular fluorescent contrast agents. It is suitable for functional imaging, optogenetics and glutamate uncaging experiments. In addition, second harmonic generation can be used to study collagen, myosin and other structures in tissues such as tumors, fibrotic lung or liver, and cardiac or skeletal muscle. The platform also supports deep imaging in thick specimens, organoids, spheroids and transparent organs, as well as UV fluorophore imaging in lipid studies with Laurdan and Filipin.

Technical Specifications

-Automated vertical multiphoton microscope with bridge-type vertical configuration

-Full motorization with motorized sample stage and integrated joystick

-Three detection channels: GaAsP and multialkali, plus a transmitted-light detector

-Specifically optimized for infrared excitation

-Powered by a tunable pulsed laser source from 660 to 1320 nm

-Includes a fixed 1040 nm line

-Supports second harmonic generation imaging

-Capable of deep tissue imaging in thick and living samples